Microfluidics is the science of systems that manipulate small amounts of fluids (between uL and fL), using channels with dimensions of tens to hundreds of microns. The reduced physical dimensions lead to laminar flows, guaranteeing optimum control of the flow properties and high reproducibility. Droplet microfluidics relies on the production of droplets with controlled size, where each droplet can be considered a small and independent reactor flowing in an immiscible liquid. Notably, droplet microfluidics is a rapidly growing interdisciplinary field of research combining soft matter physics, biochemistry and microsystems engineering. In our group, the activities in this area follow two main themes: dynamics of droplets in confined systems, and microfluidic devices for extracellular vesicles isolation and analysis.