Thanks to their structural programmability and predictability, nucleic acids are widely employed as material building blocks to develop new artificial receptors or drug carriers. In this project, we investigate an allosterically controlled DNA biosensor, focusing on how its structure and the binding  affinity for the target are changed by the interaction with the allosteric effector molecules. Pulling experiments are carried out tethering the DNA sensors from its ends and monitoring the lifetime of its folded and unfolded states in presence of the target and the allosteric molecules. The research is funded by the Italian PRIN 2022 project “Single-molecule and Fluorescence Investigation of Dissipative Allosteric DNA-translators”.